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In this fashion a pool of genes expressed with high level of reference concordance was selected.
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To define and characterize the resultant respondent types, all participants who loaded heavily on a respondent type (>60% concordance) were selected as respondent type "loaders".
Repeated analysis in several runs showed a high degree (96.5%) of concordance, and the most robust call was selected in the case of discordance (3.5%).
Then for each pair of DV gene lists, the 200 genes with the lowest P-values were selected to calculate the concordance between two gene lists.
Around 4.5% of samples were selected for duplication and the concordance is 99.5%.
We conducted this nested series of analyses to determine if a gender difference in concordance could be reliably detected regardless of how studies were selected.
A5EΔ4 splicing exons were selected according to both transcript coverage, concordance of tissues inferred from cDNA-libraries of A5EΔ4 genes, and commercially available samples.
Several deletions were selected for Syber Green qPCR, with 100% concordance between called deletions and PCR results (Supporting Information S1).
For quality control, 10% of the samples were selected at random for repeated genotyping and concordance was 100%.
Each plate included negative controls (with no DNA) and 12 randomly dispersed samples were selected from each 384-well plate and duplicated on a separate concordance plate.
Secondly, 3% samples were selected randomly and genotyped for the same SNP by different experimenters, indicating the concordance rate of 100%.
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