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The calculations were done using Partekpro 5.0 software (Partek Inc., St. Charles, MO) The global concordance of expression levels of the three platforms can be expressed in terms of the 'correlations of correlations' coefficient described previously [ 10, 11].
To assess the concordance of expression pattern by RNA-Seq and microarrays, all genes expressed by RNA-Seq were evaluated as to whether they were identified as expressed in all microarrays, a subset of microarrays or not represented on the microarray.
A representative part of these genes exhibited a good concordance of expression changes when assessed using tailored Q-PCR.
However, validations using independent cohorts are also crucial for a biomarker study as they facilitate inter-study concordance of expression trends and signatures.
Thus, we used Spearman Rank Correlation to determine the concordance of expression values in the PolyA+ vs rRNA-depleted samples (Additional file 1: Table S6).
We also reasoned that, if such hierarchy existed, transcripts with highest concordance of expression between tissues and cell lines should also be most likely to be affected by genetic factors driving the oncogenic process including aneuploidy.
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Moderate concordance of MET expression and significant expression heterogeneity may be a barrier to the development of predictive biomarkers using MET targeting agents.
The concordance of AR expression and UPR specific gene expression was evaluated with Pearson's correlation analysis using the software R. YJA, MS, MT, XS, YJ, SZ, and HZN performed the experiments.
To evaluate the degree of concordance for expression values of probe pairs, a correlation analysis was carried out.
To examine the concordance of gene expression measurements across methods, we extracted 1,952 expression probes that showed variable weights between -0.01 and 0.01 for all methods.
Herein we quantified gene expression from 92 patients with acute lymphoblastic leukemia (ALL) in paired normal and cancer tissues to assess the concordance of gene expression in these tissues, and the relationship between germline genomic variation and gene expression.
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