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Although it is not surprising that these and multiple other known regulators of HSC function were identified, this concordance of data support the validity of our approaches and suggests that the numerous novel genes discovered here will play important roles in the regulation of HSC function under different circumstances.
While this concordance of data was also true for the vast majority of genes in the HSC vs. LSC comparison, some genes identified as downregulated by array analysis did not show significant downregulation when analyzed by qRT-PCR in an independent set of sorted samples.
Furthermore, the concordance of data entry of some clinical variables entered in both registries was evaluated.
Of 13 studies, 8 produced results supporting the reproducibility and concordance of data across different microarray technologies.
The following variables were chosen to investigate the concordance of data entries in the two registries: initial haemoglobin and blood pressure levels on arrival at the emergency department, the ISS, and mortality.
We were especially interested in the extent of concordance of data entries for some of the variables entered in the two registries, including the initial haemoglobin (Hb) and blood pressure (BP) levels on arrival at the emergency department (ED), the Injury Severity Score (ISS), and mortality.
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These data show that the concordance of DASL data with IHC data for all three receptors is very high, which is consistent with previous studies relating mRNA and IHC protein levels (Cronin et al, 2004; Gong et al, 2007).
Our data show that the concordance of DASL data with IHC data for all three receptors is very high, which is consistent with our previous published work relating mRNA and IHC protein levels [ 14].
We first validated our BCP for use in the DASL assay through concordance of DASL data with ER, PR and HER2 IHC data as well as by comparison of 152 genes in common with the HCP.
For bivariate data, coefficients of correlation based on ranks measure concordance of the data.
However, in some cases we find concordance of cytogenetic data with phylogenetic traits such as morphology, bioacoustics, and molecular data.
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