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Sensitivity of the mutants to RAL was measured in processing, strand transfer and concerted integration.
Standard concerted integration reactions were performed as described previously [16], [17].
This also explains why, in our standard in vitro concerted integration assays, MAba4 strongly impaired the IN enzymatic activity.
The antibody incubated with IN inhibits its enzymatic activity in in vitro concerted integration assays [22], [48], [49].
The activities of 3'-end processing, strand transfer (ST) and concerted integration were compared to the wild-type one.
Standard concerted integration reactions were performed as described previously [52], except that no cellular proteins were added.
Similar(28)
Based on our analysis that 87% (13 of 15) of the proviruses had a correct 4-bp direct repeat at the integration site, we conclude that the majority of XMRV integration reactions involve a concerted joining of two viral DNA ends that are spaced 4 bp apart on the target DNA.
HIV integration into the host genome is a multistep process involving: a) 3′ endonucleolytic cleavage of the 3′ end of DNA in the HIV pre-integration complex to a conserved CA dinucleotide, b) Strand-transfer involving concerted cleavage of host DNA and ligation of viral 3′ DNA to 5′ staggered site on host DNA, and c) DNA repair of gaps and DNA synthesis.
The movement away from global integration, however imperfect, comes at a perilous time when imminent man-made and natural risks require concerted, globally coordinated responses.
As a result, the vote may delay concerted pro-growth steps by central banks and governments around the world, as well as the hard choices within Europe over deeper integration that are likely to prove necessary in the long run.
Surprisingly, the fine words prompted concerted action.
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