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When expressing cellulases in plants, it is important to consider all factors concerning the expression levels and relative activity of the enzyme in relation to its localization within the plant.
Concerning the expression levels of the HER receptors (HER1, HER3 and HER4) and the cell cycle proteins cyclin A, D1, E and p-cdc2, no change was observed, HER2 is shown as example.
The high-throughput of microarray technology has led to explosion of data concerning the expression levels of the genes but most of statistical methods such as fold change and t-test identify genes with significant changes.
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Therefore, we analyzed our data set concerning the expression level of S100 proteins with respect to their association with the development of distant metastases.
In this case an accurate estimate of Ibg is crucial to draw reliable conclusions concerning the expression level; estimates based on the intensity distribution over the whole chip suggest that this is the case for roughly a quarter or half of the probes [ 3].
Our results show differences concerning the expression level of each gene during experimental challenge in controls in families A and P. Nevertheless, at 4 hpi down-regulation of five genes was reported in infected oysters from family A in comparison with family P.
Concerning the expression studies, we measured ADIPOR2 expression only at mRNA level and it is not known if this difference is reflected in expression at protein level.
The authors should show data concerning the relative expression levels of the fusion proteins and mention how this may affect their results and interpretation.
The response of the authors to this concern is not acceptable, for example, the expression levels of Atgs are not direct indicators of autophagic activity.
The expression levels were Lg transformed.
Expression levels of β-actin and GAPDH were used to normalize the expression levels.
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