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The fusion event identified in Monosiga, resulting in a combined "glutamine hydrolysing (not ammonia-dependent) carbamoyl phosphate synthase" (EC 6.3.5.5) and "aspartate carbamoyltransferase" (EC 2.1.3.2), appears to be common among fungi, metazoa and some bacteria, and concerns enzymes that participate in subsequent steps of the pyrimidine biosynthesis pathway.
Subsequent steps will be more tentative, though.
All subsequent steps were performed at 4 °C.
All subsequent steps were carried out at 4 °C.
The subsequent steps of were performed as previously described18.
All subsequent steps were performed on ice or at 4 °C.
The subsequent steps are proposed.
Here's hoping that subsequent steps bring long-needed change.
The subsequent steps were the same as the control group.
The subsequent steps of the preprocessing further improve the accuracy.
The subsequent steps were similar as mentioned above.
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