We thank Johannes Kühn for the laboratory support and Theo Peschke as well as Pascal Baumann for the support concerning expression optimization.
MPSS has been used for whole genome transcription analysis in the last decade and has generated abundant data concerning expression in many organisms (Vega-Sanchez et al. 2007; Simon et al. 2009).
For whole cell biotransformation assays concerning expression optimization of the hydantoinase, the harvested cells were thawed on ice, washed twice with catalysis buffer and resuspended in the same buffer in a ratio of 10 mL buffer for 100 mL of harvested culture.
We provide additional evidence of the existence of core+1/ARFP in vivo and new data concerning expression of HCV core protein.
However, since no data were available concerning expression of FcεRI on enterocytes to date, we screened the intestinal mucosa of patients with gastrointestinal pathologies and controls, as well as intestinal epithelial cell lines for FcεRI expression.
Our results concerning expression of the Kv1.1 mRNA and protein support a key role for the Kv1.1 mutation in the abnormal function of β-cells from mceph/mceph mice.
