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Similarly, collagen (0.5%) and sebacic acid at different concentrations were mixed in the presence of 20 ml of water and stirred at 4°C to obtain a clear homogenous solution.
Briefly, aptamers (A1 or A2) of different concentrations were mixed with 50 μl AuNPs thoroughly and allowed to react at room temperature for 25 to 30 min. The above solution was incubated at room temperature for 10 min following addition of 50 μl PBS.
Complementary oligonucleotides referenced in "Online Supplementary Materials S1" (50 µM final concentrations) were mixed with 10X Oligo Annealing Buffer (Invitrogen) heated 95°C for 4 minutes and allowed to cool at room temperature for 10 min. Diluted (10 nM) dsDNA were subsequently cloned in XhoI/NotI restriction sites in the pSI-Check2 (Promega).
Samples with known LAP concentrations were mixed with the conjugated microbeads and the resulting fluorescent signal was analyzed by flow cytometry (Fig. 2B).
Yeast α-glucosidase (0.5 unit/mL) 20 µL, 0.1 M phosphate buffer (pH 6.9) 120 and 10 µL of test sample dissolved in DMSO at various concentrations were mixed in a microplate well.
Anionic lipids, PS, PtdIns, and various phosphorylated PtdIns's at physiologically comparable concentrations were mixed with other lipids at ratios and identities corresponding to the composition of synaptic vesicles and Table 1.
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The DHA N/O/W emulsion at varying concentrations was mixed with 2% erythrocyte solution in equal volume.
NaCl aqueous solution (2 mL) with certain concentrations was mixed with a solution of CDs (0.05 mg/mL, 2 mL).
The blue solid line shows the resistance versus time curve as various CO concentrations are mixed with the flowing dry air of the test chamber.
A volume of 500 μL of each sample at different concentrations was mixed with 500 μL of 99.5% ethanol and 125 μL of 0.02% DPPH in 99.5% ethanol.
Briefly, 200 μL of each extract solution of different concentrations was mixed with 1 mL of Folin-Ciocalteu reagent (1 : 10 diluted with H2O) and 800 μL of Na2CO3 (75.05 g/L).
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