Sentence examples for concentrations were established using from inspiring English sources

Free Ca2+ concentrations were established using an EGTA-buffering system.

Protein concentrations were established using a protein estimation kit (Bio-Rad, Hercules, CA, USA).

All primers were HPLC purified, and optimal concentrations were established using serial dilutions of reference cDNA in preliminary experiments.

RNA concentrations were established using a NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and the RNA integrity number (RIN) was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Massy, France).

The relationship between specific viscosity and concentration is established using rheometer and applying Zimm model (dilute solution), Rouse Zimm model (semidilute unentangled solution), and Doi Edwards model (semidilute entangled solution).

Protein concentration was established using 2-D Quant (GE Healthcare).

The protein concentration was established using a BCA technique (Thermo Scientific), and 30 40 μg of protein were then separated by electrophoresis on a Bis-Tris NuPage gel.

Protein concentration was established using the Coomassie (Bradford) Protein Assay Kit (Thermo Scientific), briefly 10μl of crude homogenate was diluted 1 50 and read in triplicate at 595nm using a FlexStation 3 (Molecular Devices).

The concentrations of cytokines were established using the coefficient of linear regression from values obtained on the standard curve.

After reaching stable contraction, cumulative concentration-response curves were established using ACh (1 nM–10 μM; Sigma), GTN [1 nM–100 μM; Nitropol® ampoules (4.4 mM); G. Pohl-Boskamp GmbH, Hohenlockstedt, Germany] or 2,2-diethyl-1-nitroso-oxyhydrazine (DEA/NO; 1 nM 10 μM; Alexis Corp., Lausen, Switzerland).

Concentrations of β2-m were established using immunoturbidimetry in CSF and corresponding sera in Synchron LX-20 analyser.