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The 3 concentrations were decided that the difference in between two consecutive levels is the same.
PQ treatment concentrations were decided upon in preliminary life history assays; PQ levels that dramatically reduced or prevented nematode development to maturity under otherwise standard conditions were avoided.
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APN (5 μg/mL: this concentration was decided, based on the previous publications 17, 18 or phosphate buffer saline (PBS) for an additional 18 h).
Once the thrombin concentration was decided, the purified protein was mixed with proper amounts of thrombin and dialyzed in low salt buffer (20 mM Tris, 100 mM NaCl, pH7.5) at 4°C for 16 hours.
On the basis of the evaluation of trial batches, concentrations of the polymers were decided.
Drug concentrations and pulse schedule were decided based on changes in cell morphology and resistance; a dynamic pulse schedule was used here according to our previous experience with this cell line.
For the assay of conjugative effect, sublethal concentrations of SL and cefaclor were decided to be 500 μg/mL and 50 μg/mL, respectively.
Based on the results of MIC determination experiment, the sublethal concentrations of SL and cefaclor were decided and used for further experiment.
The concentrations of MGO, FA, CG, and talc were decided based on previous reports [ 7– 16].
When the concentration of AgNO3 was high enough, the reductive amounts were decided by reducing agent.
Accordingly, in this work to improve the corrosion resistance of stainless steel in NaCl solutions with different concentrations, it was decided to make use of the useful properties of manganese oxide on SS316L.
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