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Protein concentrations were assessed using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific).
Protein concentrations were assessed using the Pierce ™ BCA Protein Assay Kit
Nickel concentrations were assessed using colorimetric detection at 660 nm.
Soluble biomarker concentrations were assessed using MILLIPLEX MAP Human Cardiovascular Disease CVDD) Magnetic Bead Panel 1 - Cardiovascular Disease Multiplex Assay.
Cellular ATP concentrations were assessed using the CellTiter-Glo® Luminescent Cell Viability Assay as per the manufacturer's instructions (Promega); for cardiac microtissues 40 µl media was removed prior to the addition of 40 µl CellTiter-Glo® reagent.
At each site, nutrient concentrations were assessed using field chemistry kits to measure nitrate-N and phosphate-P.
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Spatial variability of the POP concentrations was assessed using an IDW spatial GIS model analysis.
The correlation of gene expression with cadmium concentrations was assessed using linear regression.
Any anti-inflammatory effect of synovial ASA concentrations was assessed using an ASA EC50 (half maximal effective concentration) determined in equine whole blood.
Bisulfite DNA quality and concentration were assessed using the NanoDrop and, if required, samples were concentrated to approximately 50 ng/ul using a SpeedVac (Thermo Electron Corporation, Waltham, MA, USA).
The impact of pH and loading buffer salt concentration were assessed using robotically operated 20 μL packed pipette tips.
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