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The cell concentrations were ascertained by direct counting using Trypan blue.
A significant positive relationships between baseline values of plasma ADM and plasma NA concentrations were ascertained for older and younger groups of subjects (r = 0.76, P < 0.001; r = 0.62, P < 0.01; respectively).
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The axial and radial profiles of the solid concentration were ascertained by sampling the suspension.
DNA purity and concentration was ascertained by use of a Nanodrop™ 1000 spectrophotometer.
Before each experiment, eGFP-CaM was thawed and centrifuged for 10 min at relative centrifugal force 20,800 × g and its concentration was ascertained using FCS.
The lysate concentration was ascertained and was equally diluted in immunoprecipitation dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mmol EDTA, 16.7 mmol Tris HCl, 167 mmol NaCl, pH 8.1).
RNA concentration and quality were ascertained by using the NanoDrop® ND-1000UV spectrophotometer and Agilent 2100 Bioanalyzer (microcapillary electrophoresis on RNA 6000 Nano LabChips, Agilent Technologies).
Furthermore, significant correlations were found between exercise-induced changes in cardiovascular indices and those in the hormone concentrations: namely positive correlations were ascertained between plasma ∆ET-1 and ∆ΜΑP, and ∆TPR; negative relationships between ∆ADM and ∆SV, ∆TPR or ∆PEP/LVET ratio.
Based on the mix design parameters established within the study, concentrations of NaOH and Na2SiO3 were ascertained that are advantageous for constructability and early age mechanical behavior.
All urine samples were diluted 1 1 or more for the ELISA, and the concentrations of the respective molecules were ascertained from standard curves constructed using manufacturer-supplied standards.
RNA quality, concentration and integrity of samples were ascertained using Agilent 2100 Bioanalyser (Agilent Technologies, Foster City, CA).
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