Cu and Zn in different molar concentrations were loaded using electroless deposition over the prepared γ-alumina support.
Briefly, same amounts of drops containing GFP-expressing S. typhimurium with different normalized concentrations were loaded into the open-cylinder type reservoir and the numbers of cells were calibrated by analyzing the fluorescent intensity.
Equal protein concentrations were loaded based on quantification with the BCA™ Protein Assay (Pierce, Rockford, IL) following the manufacturer's procedure.
Equal protein concentrations were loaded onto 10% Tris HCl gels and subjected to SDS-PAGE.
Equal protein concentrations were loaded in each lane as confirmed by Ponceau staining of the blot membrane.
Enzyme-linked immunosorbent assay (ELISA) for β Amyloid (42) (Rat) ELISA Kit (Wako, Japan) was performed according to the manufacturer's instructions, except that equal protein concentrations were loaded into each well.
The following concentrations were loaded to the gels: 10 μg of each cancer extract, 20 μg of the ovarian lysates according to the manufacturer (www.prosci-inc.com), and 100 μg of the human cerebrum and cerebellum extracts.
10 μl samples of the P60A mutant of R16 α-spectrin, containing a range of urea concentrations, were loaded into standard-treated, zero background MST-grade capillaries to increase signal to noise ratios.
Fourteen samples at a range of concentrations were loaded in cell assemblies with standard 12 mm Epon double-sector centerpieces, installed in an 8-hole rotor, and placed into the rotor chamber for exhaustive temperature equilibration at 20 °C, typically for 2 3 h, followed by acceleration to 50000 rpm.
A final amount of 0.5 ml at 0.5 mM total lipid concentration was loaded into the calorimeter, and up to six heating scans were performed at 45 °C/h between 10 and 60 °C for all samples.
