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Determination of the adsorption equilibrium concentrations was determined by UV vis spectrophotometry analytical method.
Conditioned media were collected, concentrated using Amicon Ultra centrifugation filter (MWCO 10K, Millipore), and protein concentrations was determined by Bradford assay (Thermo Scientific; Rockford, IL) according to the manufacturer's instructions.
A good reproducibility of the VFA concentrations was determined and standard deviations were below±1% of the measured concentration.
Thus, a standard calibration curve for different RNA concentrations was determined so that cell numbers could be inferred from the assay.
Additionally, the efficiency of sorbent regeneration using HNO3 at different concentrations was determined.
Influence of initial dye concentrations was determined using 5, 10, 15, 20, and 25 mg/L of RR31 dye.
Similar(29)
Their concentrations were determined spectroscopically.
Protein concentrations were determined using the BCA Protein Assay Kit Thermo Fisher Scientificc).
Protein concentrations were determined according to Bradford.
Protein concentrations were determined using the BCA Protein Assay Kit Pierce Biotechnology Rockfordrd, IL, USA).
Antibody concentrations were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific).
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