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Spatial variability of the POP concentrations was assessed using an IDW spatial GIS model analysis.
The correlation of gene expression with cadmium concentrations was assessed using linear regression.
Any anti-inflammatory effect of synovial ASA concentrations was assessed using an ASA EC50 (half maximal effective concentration) determined in equine whole blood.
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Nickel concentrations were assessed using colorimetric detection at 660 nm.
At each site, nutrient concentrations were assessed using field chemistry kits to measure nitrate-N and phosphate-P.
The measured concentrations were assessed using the guidance values defined by the German Act for the Regulation of Surface Waters of 2011 [16] and the German Working Group on Water Issues (LAWA) [17].
Protein concentrations were assessed using BCA assay from Pierce.
Protein concentrations were assessed using a Picodrop™ (Picodrop Limited, Saffron Walden, UK).
CK-B concentrations were assessed using a non-competitive sandwich ELISA.
DMPC concentrations were assessed using the colorimetric Phospholipids C kit purchased from Wako Chemicals USA, Inc. (Richmond, VA), following manufacturer's protocol using choline as a standard.
Supernatants were transferred to fresh tubes and protein concentrations were assessed using the Bradford method (Bio-RAD protein assay, BioRad, Hercules, CA).
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