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The anti-Neu5Gc antibody or Chicken IgY concentrations used on human sections were each at ∼5 micrograms per ml on frozen or on paraffin sections (1∶1000 or 1∶500 respectively - when detecting larger amounts of Neu5Gc in animal tissues it is possible to use dilutions of 1∶10,000 or 1∶20,000).
Of note, RNA input concentrations used on this platform should be maximized whenever possible, and cDNA dilution factors should be kept to a minimum.
We also wanted to use the same drug concentrations used on larvae in these experiments so that we could directly compare cellular responses and organismal responses in the same system.
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Apparently, different Rhodococcus biosurfactant structures formed, depending on the concentration used, on the polystyrene surface would modify differently its physicochemical properties, thus leading to diverse anti-adhesive effects.
TPEN was prepared in DMSO and the final DMSO concentration used on cells <0.3%.
There is contention, however, whether the tracer concentration used on patients is sufficient to adequately label NFTs and/or other pathologies.
Increasing concentrations of the inhibitors were assayed (Table 2) and sublethal concentrations used to assess their effect on PrE organisation.
The actinide concentrations used were based on extraction from 30 year cooled spent fuel and mixed with natural uranium in 4.75% actinide MOX fuel.
Breakpoint concentrations used are based on long-term studies within the Health Protection Agency Laboratory of Enteric Pathogens.
The analogue concentrations used were based on the DHEA concentrations of serum levels found in humans and other species and were chosen to approximate analogue levels in vivo.
Those results are based on only the six BSC plants for which concentration measurements were taken, but the modelled concentrations used depended strongly on extrapolation from NSF measurements; and only 60 lung cancer deaths (compared with 184 from NSF).
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