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This observation was without exception in all inhibitor and glutamate concentrations used not only in this but also in our previous studies of AMPA receptors in the absence and presence of other inhibitors.
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Notably, treatment of both cells types with M βCD at the concentrations used did not significantly interfere with cell viability, as determined using Trypan blue (data not shown).
Short exposures to copper and cadmium at the concentrations used did not affect C. crambe settlement compared with SW control, and no effect on consecutive survival of juveniles was observed.
It has to be noted that all the acid concentrations used are not enough to dissolve the ZnO structures, and no evidence of degradation due to any chemical reaction after contact with acidic pH solution was experienced.
Heavy metals in concentrations used did not appear to affect hatching of wild-type or mutant strains.
It is shown in Figure S1 that these reagents at the concentrations used did not affect cell viability.
As controls, direct addition of CFTR inhibitor/antibody or acetazolamide to the bacterial culture (1×105 CFU) at the concentrations used did not affect the growth of E.coli, excluding direct effect of the inhibitors and antibody on the bacteria.
Preliminary results using the NB-like precipitation system developed in that study [13] suggest that the addition of excess calcium and/or phosphate to serum, even at the high concentrations used, may not have exhausted the full inhibitory (and seeding) capacity of the serum studied and that only a fraction of the total inhibitory components has been consumed during this process (not shown).
We found no evidence of dynasore adversely affecting the parasites themselves at the concentrations used (data not shownFigure S1????) Previous studies using dynasore have shown that dynamin is involved in the infection of mammalian cells by papillomavirus [25] and in phagocytosis by Sertoli cells [15].
Furthermore, the drug concentrations used did not affect viability.
Whereas the N-lobe at all concentrations used could not detect any fluorescence signal in flow cytometry scan profile.
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