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In addition, some concentrations used in the in vitro assays were quite low or may not have reflected physiological concentrations; use of higher concentrations may have led to different results [ 41].
For serum concentrations of oxycodone and the metabolites and the ratio oxymorphone/oxycodone these were: oxycodone total daily dose, use of CYP3A4 inhibitors or inducers, sex, time since last oxycodone dose, the number of medications other than opioids used in the last 24 h, albumin serum concentrations, use of steroids, BMI and glomerular filtration rate.
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The concentrations used of the methanol extract and fractions were from 25 to 200 μg/mL, each assay was performed in triplicate.
The second explanation concerns the concentration used of the quenchers.
Concentrations used to built calibration curve of betulinic acid.
At the concentrations used, none of the MVA-derived products altered the proliferation (data not shown).
LPC has non-specific detergent properties at high concentrations, preventing use of a concentration that generated maximum TRPC5 activity.
Over the range of concentrations used, the pH of the MB solutions was constant at 6.2.
We added concentrations of the four THMs to get TTHM concentrations, using imputed concentrations of the individual THMs for nondetects.
LaF3 Tb nanoparticles assessed in 3T3 cells were determined using various concentrations by use of the WST-1 assay.
The shown ammonia concentrations for use of nitrite are an extrapolation of the model data.
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