The concentration of C-terminally truncactinactin was determined densitometrically as described in [15].
The concentration of C-terminally truncactinactin was determined by densitometry of actin bands separated on SDS gels.
For example, high concentrations of truncated mRNA fragments could saturate exonucleases that process these fragments into mononucleotides19.
Rat TrxR1 (5 nM), human wild-type Trx1 (10 µM), kindly provided by Arne Holmgren, Karolinska Institutet, and NADPH (200 µM) were incubated in TE buffer containing 1 mg/ml BSA with different concentrations of truncated rat TrxR1 as a SecTRAP preparation, added as indicated in the text.
The reaction mixture in a total volume of 20-µl contained 0.3 µg of supercoiled Rf I ΦX 174 DNA, 10 mM Tris-HCl buffer pH 7.5, 5 mM MgCl2, 0.1 mM EDTA, 15 µg/ml bovine serum albumin, 50 mM KCl and various concentrations of truncated specimens or wild type proteins.
Once the mRNA poly(A) tail is removed, reverse transcription reaction will not proceed, resulting in low concentrations of truncated transcripts (see Figure 1b).
The critique concerns the shape of velocity, temperature and concentration profiles which are truncated due to small calculation domain used during the numerical solution procedure.
