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Trials are conducted at various concentrations to observe effects and to obtain the concentration that caused maximum deviation from the controlled condition.
The test compounds were used in 1, 3, 10, 30, 100, 300 and 1000 µM concentrations to observe their inhibitory effect.
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The experiments were conducted by varying Fe(II)/H2O2 molar ratio at a fixed pH and initial dye concentration to observe the optimal ratio.
In this study, we performed a repetitive cultivation with a stepwise increase in ethanol concentration to observe the adaptation process of S. cerevisiae to a high ethanol concentration.
Interpretation of those experiments was limited by the questionable specificity of these agents and their toxicity at the concentrations needed to observe any protective effect.
Preliminary experiments were performed to determine the lowest PmHS2 single-action transferase and UDP-sugar concentrations needed to observe activity.
Conversely, for mutants with slow cleavage rates (<∼0.1 s−1), kcat cannot be readily measured due to the long time-points and high substrate concentrations required to observe cleavage.
Henriksen et al. [ 27] have previously shown that the high concentrations needed to observe inhibition in the cell-based acridine orange assay can lead to unclear results.
Using various inhibitor concentrations, we failed to observe any interference with BRCA1 downregulation by MMS (data not shown).
A sample was collected from the aqueous phase outlet after a regular interval and analyzed for the concentration of solute to observe the steady-state condition.
However, a higher concentration was required to observe its effect (SI Figure S9).
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