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This protocol allowed the concentrations of target proteins in different compartments of the cells to be determined and changes in concentrations to be analyzed under specific perturbations at different times.
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Typically, approximately one-third of the cultures reached a sufficient cell concentration to be analyzed for the size selections in every round.
The option of dilution depends on the concentration of substance to be analyzed and the biosensor sensitivity: if the biosensor can identify the target substance in concentrations, which are dozens of times lower than those actual in the samples, the latter should be diluted to decrease the content of interferents and thus improve the array accuracy.
Medium was then removed and replaced with fresh medium containing 0.5 mM IBMX, with or without 10 μM forskolin and various concentrations of the peptides to be analyzed.
However, the stochastic recurrence relation for the calcium concentration alone is simple enough to be analyzed and we discovered that it follows a gamma distribution with shape parameter (lambdatau_{mathrm{ca}} +1).
Unfortunately, the sample size was relatively small for the independent effect of ALI/ARDS on plasma cf-DNA concentration to be robustly analyzed.
CAIT leads to concentration depletion profiles, which can be analyzed quantitatively by means of the Nernst-Planck-Poisson theory.
The effects of feed concentration, generator temperature, HPP pressure, LPP pressure, and concentration range are analyzed to provide guidance to NCAHT design.
Untreated cells (negative control), cells exposed to etoposide (50 μM), an inducer of LMP, or to different acetate concentrations were analyzed at different time points.
The dichotomous aflatoxin concentration variable was analyzed to compare the odds of exposure to aflatoxin at levels > 20 ppb by market location, maize history, and vendor type.
Differences in the CBF3 and COR47 transcriptional patterns in response to different ASA concentrations were analyzed using SPSS 16.0 software.
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