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We also showed that solutions less than 4 μM decreased in concentration after 2 months when stored in the dark at 4°C, while all concentrations stored at room temperature, whether protected from light or not, decreased in concentration after 4 months of storage.
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Stock solution was further diluted in dichloromethane to obtain working standard solutions of various concentrations and stored at 4°C until use.
Stocks were prepared at 10 000 × reported peak serum concentrations and stored at −20 °C.
The media was retained to measure lactate/pyruvate concentrations and stored at −20°C.
Inhibitors were all purchased from Sigma-Aldrich, dissolved in manufacturer's recommended solvents to stock concentrations and stored at −20°C.
Finally, the obtained tryptic digests were diluted to the target concentrations and stored at −20 °C refrigerator before use.
Samples of solutions of 4 μM, 8 μM, and 16 μM capsaicin concentrations were stored at room temperature and protected from light, and room temperature exposed to light.
In pool B there was a significant difference between SAA concentrations when stored at 4°C and 22°C respectively, but no similar trend was seen in the two other serum pools.
Samples (1.5 mL) from each cuvette were fixed with 60 μL glutaraldehyde (25% final concentration) and stored at −20 °C until flow cytometry analysis.
The fluorescently labelled HCV110-131 sequence (f-HCV110-131-t 5′-FAM-CGGGAGGGGGGGUCCUGGAGGC-Tamra-3′) was dissolved to a 0.1 mM stock concentration and stored at −20 °C.
Took an aliquot to measure the DNA concentration and stored at −20 °C. .
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