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Amyloid fibril formation propagating as β-sheets at the concentrations stated herein was therefore confirmed in R10 media.
Phorbol ester and diacylglycerols were pre-incubated with L. major at 27°C (or T. brucei at 37°C) for 15 min at concentrations stated in the respective figures.
For testing Opa loop-specific antibodies, Ablinear or Abcyclic were serially diluted in minimal essential medium (MEM) (final volume 150 µl), and 50 µl of diluted NHS or BBS were added to each well to achieve the final serum concentrations stated above based on a final 250 µl volume.
Other reaction components were at concentrations stated in the text.
All concentrations stated are the final drug concentration in the recording chamber.
All drugs were applied by bath perfusion at concentrations stated in the text.
Similar(34)
The reduced state vector obtained by any of these techniques contains a subset of the metabolite concentrations (state vector components) of the full model.
The calculated mean was compared to the concentration stated on the drug insert.
ESAT-6 (20 or 40 μg/ml as stated), PBS vehicle or control protein was then added at the concentration stated in text.
Nonetheless, solubility issues arose at high Ce4+ concentrations (state of charge > 60%) and elevated temperatures (>40 °C).
All subsequent experiments were therefore carried out using these concentrations unless stated otherwise.
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