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The set-up has high sensitivity, with the quantification of 3 4 μM concentrations shown to be routine.
Whether they will eventually accumulate in the high concentrations shown to have an impact here is unclear.
LY294002 was effective at 5 and 10 μM, concentrations shown to potently inhibit PI3K activity in human ASMCs (17).
In addition, we showed that mCII expression in astrocytes is insensitive to dopamine treatment, even at concentrations shown to kill neurons.
To ensure that the duration of knockdown was optimal for the observed effect, a separate experiment utilizing pharmacological inhibition was performed, using PP2 and SU-6656 at concentrations shown to inhibit kinase activity of c-Src (Fig. 3 D ).
In this instance, the technique that we used was therefore only quantitative for studying levels of acetic acid, which have been shown to stress yeast strains [ 15], but not at concentrations shown to be inhibitory (1.6 g/L).
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Fluorescence microscope showed that cells treated with all As-HAE concentrations showed to be similar to the control.
The sensor also revealed biphasic response behavior towards dynamic changes in acetate concentration shown to be strongly dependent on prior exposure to low acetate concentrations.
As shown in figure 1A, addition of 100 nM BI 2536, a concentration shown to arrest most cell lines maximally [4], resulted in a small increase in rounded up cells within 24 hours, indicative for a mitotic arrest.
In experiments focusing on profibrotic responses in fibroblasts, TGF-β at 10 ng/ml, a concentration shown to be maximally effective and non-toxic in prior studies, was used.
5×105 tumor cells were treated either with 1.800 ng of 5-Aza-2-dC (2,5 µM), a concentration shown to have therapeutic effect in vitro, or with 1×109 erythro-magneto-HA virosomes containing 10 times less 5-Aza-2dC (180 ng).
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