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The concentrations of the variables considered to be optimum by 'one-variable-at-a-time' method (glucose 4%, beef extract 1.25%) were actually higher than that actually required for maximum enzyme production.
The concentrations of the variables used in the formulation of the tablets of bisoprolol fumarate were decided on the basis of trial batches and their evaluation.
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A positive coefficient indicated that the higher concentrations of the variable is best for increasing Cr VI) removal and residual tannic acid, whereas negative values indicated the vice versa (Pulimi et al. 2012).
Saturation curves were defined by assaying enzyme activity at different concentrations of the variable substrate or effector with saturating levels of the other components.
Depending on the outcome of these analyses, the data were then fit to eq 5 to determine relevant Ki values modifying the KM of each substrate where [S]V and [S]C are the concentrations of the variable and constant substrates, respectively, and the modifying inhibition factors β1 and β2 are 1 + [NAD+]/ Ki′ and 1 + [NAD+]/ Ki″, respectively.
The optimal concentration of the variables for maximum PYC production was evaluated using a five-level three-factor central composite design (CCD).
Optimum concentration of the variables for enhanced chitinase production were 7.49, 4.91, 0.19 and 5.50 (g l−1) for chitin, colloidal chitin, KCl and yeast extract, respectively.
Optimum concentration of the variables for enhanced CDA production determined by CCD were yeast extract (X1) 3.165 g l−1, peptone (X2) 4.754 g l−1, (NH4 2SO4 (X4) 0.255 g l−1 and MgSO4 (X5) 0.535 g l−1.
The coded levels and the exact concentration of the variables used in different formulations are shown in Table 4.
Response surface methodology (RSM) was used to investigate the optimum concentration of the variables (concentration of glucose, potato, and bran extract) on the maximum yield of mycelial biomass growth and EPS production from ABM.
The concentrations of these variables depend on diet and the environment and are thus subject to repeated dynamic changes.
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