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Optimization of the growth medium was carried out using response surface methodology, aiming at optimizing the concentrations of the key components.
The theoretical values of the parameters like the maximum concentrations of the key compounds (Xmax, PHBmax and Crymax) agree with the experimental and the reported ones (Tables 2 and 3, Additional file 1: Figures S3 and S5).
On the other hand, special features of the sidechain structure can affect the concentration levels of PAHs by increasing the concentrations of the key intermediates including the benzyl radical and phenylacetylene.
After identifying effective nutrients, Central Composite Design (CCD) was used to develop mathematical model equations, study responses and establish the optimum concentrations of the key nutrients for higher growth and azadirachtin production.
Instead of the previously reported simple linear relationship between the concentrations of the key components of maize wine in the final product and the number of treatments or the dosage, various types of non-linear behaviours were observed.
After identifying effective nutrients, response surface methodology was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally.
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The regenerated stream consumption of simple networks can be predicted easily when the regenerated concentration of the key contaminant changes.
Following the PBD, RSM is usually used for further optimization to detect the optimal concentration of the key components obtained from the PBD.
The ISS includes information on the concentration of the key toxic chemical, the primary environmental medium of the exposure pathway, and the size of the population at risk.
The intracellular concentration of the key signaling ion such as calcium (Ca2+) depends on electrical gradients driven in turn by sodium (Na+) and potassium (K+) channels.
Second, the stochastic variation in expression level must have a large enough deviation from the mean to cause the concentration of the key protein concentration to dip low enough to trigger positive feedback from the disease state.
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