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We attempted to limit these potential over-expression side effects by injecting much lower concentrations of the expression vectors (ranging from 10 20 ng/µl).
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We began by examining the effect of varying PWM concentrations on the expression of SaCD79α and IgM, IgW and IgNAR using the methods we had previously developed [31].
However, the amounts of APC used were high, above steady-state concentrations (0.5 20 μg/ml), and the effect of APC in therapeutic concentrations on the expression of platelet receptors has not yet been studied systematically.
Therefore, further animal studies should be done to validate the effects of high glucose concentration on the expression of MMPs and the regulation of type I and III collagen in tendon cells.
However, independent of the general growth response there could be still a possible effect of substrate concentration on the expression level of photosynthesis genes as assumed by Kolber et al. [1] and Cho et al. [17].
However, few studies have investigated the effects of high glucose concentration on the expression of MMPs in tendon cells.
This example is only one illustration of the influence of weighted regulator concentration on the expression level of a target gene.
As tyrosine hydroxylases contain an iron atom embedded in the structure that is required for catalytic activity, we increased the Fe2+ concentration of the expression media by the addition of ferrous ammonium sulphate to ensure that all the recombinant enzyme would contain Fe2+ (63 65).
Instead, they measure merely the concentration of the expression level and, hence, provide indirect information about molecular interactions only.
With further exposure to increasing concentrations of TET, the expression of acrAB-tolC increases as the organism becomes more and more resistant to the antibiotic.
Following exposure to three concentrations of TCDD, the expression levels of the 11 genes were calculated relative to unexposed controls.
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