Concentrations of the compound have been increasing in the atmosphere, and because of the compound's relatively short lifetime, it is not regulated now under the Montreal Protocol.
Results showed that subinhibitory concentrations of the compound could significantly inhibit biofilm formation in V. cholerae in a concentration-dependent manner.
In an extensive study of BPA retained by Canadians' bodies that was published in August, Statistics Canada, a government agency, found that almost no one escaped the chemical and that the highest concentrations of the compound were found in teenagers, with younger children a close second.
Different concentrations of the compound under test (10, 25, 50, and 100 μM) were added to the cell monolayer.
The final concentrations of the compound in the test tube were 0.4, 0.8, 1.2, 1.6, and 2 mg/mL, respectively.
Different concentrations of the compound under test (10, 25, 50, and 100 μΜ) were added to the cell monolayer.
The final concentrations of the compound in the medium containing plates ranged from (0.1 20 mg/mL) for butanolic extract and (0.5 15 µg/mL) for pure compound.
While fish should need more and more TMAO to survive ever greater depths, higher concentrations of the compound also draw in more and more seawater through osmosis, the process by which cells regulate their water content.
To determine the minimum bactericidal concentration (MBC), aliquots from clear wells containing increasing concentrations of the compound were plated onto antibiotic-free agar plates, and the lowest concentration at which no visible growth was observed was reported as the MBC.
However, higher concentrations of the compound were very effective in blocking TRPM7 channel activity (Fig. 2C,D).
Not all studies have had a successful treatment effect despite high CNS concentrations of the compound administered [51].
