In some biopsies, there was a concentration of staining at the apical surface of the epithelium.
The kit is meant for use on a single defined bacterial species in a concentration of staining solution that was determined following a thorough calibration procedure.
The boundary flux (N bc ) defines the boundary condition for the diffusion model, which is a parameter affected by the concentration of staining solution used.
At short intervals after injection of all doses there is a concentration gradient of staining intensity of the antibodies with increasing distance from blood vessels within tumors that strongly express the target receptor.
Alternatively, this difference may also be caused by higher concentration of FITC-Galacto-RGD2 (>5 μM) than that of integrin β3 antibody (2.5 μg/mL or ∼12 nM), as illustrated by the concentration dependence of staining kinetics for human cancer tissues (Supporting Information Figure SI1).
The sections were first treated with 0·5 M ethylenediamine tetraacetic acid (EDTA) for 3 min, dehydrated with graded concentrations of ethanol, stained with HistoGene Staining Solution [LCM Frozen Section Staining Kit (Arcturus®; Applied Biosytstems, cat. no. KIT0401NS, Foster City, CA, USA)] for 45 s.
Antibody specificity was confirmed based on negative staining with an appropriate non-immune antibody, a concentration-dependent titration of staining intensity, a lack of extracellular staining, and specific competition of antibody binding by a pRKIP peptide (see Figure S1 in Additional file 1).
Special attention must, therefore, be paid to the use of excess Nile red concentrations for staining neutral lipids.
Since proteoglycans have many closely placed negative radicals, sections with high concentrations of proteoglycans, stained with toluidine blue, determine closely arranged and interacting molecules of this stain, replacing the blue with a violet coloring.
Overall, microsphere distributions closely followed the dye patterns, with microsphere concentrations at all depths directly related to the intensity (or concentration) of dye staining.
