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The white crosses indicate the concentrations of samples that were loaded into capillaries for SAXS measurements.
14C concentrations of samples with lower CFCs were measured, and corrected for addition of 14C-free dissolved inorganic carbon.
20 h after incubation, the cells were treated with various concentrations of samples.
Concentrations of samples showing 50 % reduction in cell viability (i.e., IC50 values) were then calculated.
Three different concentrations of samples (20, 100, 200 μg/mL) were added to cell culture wells.
For PNPASE purified from HEK mitochondria, two concentrations of samples were loaded.
The parameters optimized were current density, type of electrodes, % bias, concentration of supporting electrolyte, and concentrations of samples and reagents.
The MSCs in the samples group were injured and incubated for 24 h in DMEM in the presence of various concentrations of samples.
At the higher tested concentrations of samples, the matrix effects were significantly reduced for both cathinone and mephedrone by using standard addition technique.
Finally, the cellular ATP concentrations of samples were determined by the Clean Sense TM Surface Hygiene Test Kit LEY U Biotechnology, Shanghai, China).
A 100 μL of 100 μM Hg2+ solution was added into 900 μL lake water, the resulting solution was 10 μM, which was further diluted a series of different concentrations of samples with lake water.
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