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Different concentrations of gallic acid were prepared in 80% of methanol.
Calibration curve was constructed with different concentrations of Gallic acid (1 12 μg/mL) as the standard.
The absorbance values of different concentrations of gallic acid were plotted and a standard calibration curve (y =8.8234x + 0.1616; R2 = 0.9933) was found.
The total phenols concentration in the extracts was calculated from a standard calibration curve obtained with different concentrations of gallic acid, ranging from 0 to 600 μg mL−1.
For the calibration curve (R2 = 991), the absorbance of different concentrations of gallic acid (CAS Number 5995-86-8, SigMalaysiach, Malaysia)was read and the final TPC was expressed in milligram gallic acid equivalent (GAE) per gram DM [23, 24].
Known concentrations of gallic acid were used as standards.
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The linear relationship between peak height and the concentration of gallic acid was obtained over the range of 0.1 35.0 mg L−1 with the detection limit 0.081 mg L−1.
During 35 electrochemical pretreatments and square wave voltammetry sweeps, the electrochemical system provided repeatable voltammograms for five levels of concentration of gallic acid in 0.5 mol L−1 sulfuric acid, according to the AOAC acceptance criteria for repeatability.
X: The concentration of gallic acid in mg/mL.
The final concentration of gallic acid was 1 mM, unless noted otherwise.
The calibration equation was y = 0.0111x − 0.0148 (r2 = 0.9998) where y = absorbance and x = concentration of gallic acid in mg/L.
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