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30 ng of miR-206 or YY1 luciferase reporter construct, different concentrations of expression plasmid, and 30 ng of beta-gal plasmid pSV-β-Galactosidase Control Vector (Promega, Madison, WI) were transfected using FuGENE HD (Roche, Indianapolis, IN).
HAART-treated patients, either having HALS or not, showed significantly higher concentrations of expression of the three CNT mRNA subtypes with respect to controls and naïve, with distinct concentrations of statistical significance depending on the CNT subtype (Figure 2).
Co-transfections with promoters were performed with empty vector or different concentrations of expression constructs of HDAC4 as indicated.
In order to investigate the genes' capacity to confer fluoroquinolone resistance to the E. coli host, different concentrations of expression inducers were added to the Mueller-Hinton growth media used for fluoroquinolone susceptibility determinations with Etest® gradient strips (BioMérieux SA, Marcy l'Etoile, France).
Chondrocytes were transiently co-transfected with the PPARγ promoter and increasing concentrations of expression vectors that encode Sp1 or Egr-1, and at 18 hours post-transfection, the cells were left untreated or stimulated with IL-1 for an additional 18 hours.
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Significant differences were determined using biological triplicates and Student's t-test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001) Fig. 5 Effects of different sucrose concentrations on expression of Wx a and Wx b genes.
What's astonishing about Brahms's achievement in the Fourth Symphony is that this ferocity and concentration of expression is achieved not through a heightened emotional rhetoric, but through a relentless focus on supposedly "abstract" musical details.
The control and concentration of expression is in contrast to the verbal flood of much Romantic writing.
His "Kiss" (1908), with its two blocklike figures joined in symbolic embrace, has a concentration of expression comparable to that of primitive art but lacking its spiritualistic power.
For transfection of the chondrocytes, the working concentration of expression plasmids was 0.4 μg/ml, and the working concentration of siRNAs or microRNAs was 20 nmol/ml.
This confirms previous immunohistochemistry findings in the human placenta, which had localized these transporters to the syncytiotrophoblast at term, with many studies describing a high concentration of expression at the apical membrane of syncytiotrophoblast (19, 20, 26, 35, 36).
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