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The infected cells were treated with various concentrations of compound for 24 h, and the remaining levels of EV71 RNA in RD cells were determined by qRT-PCR.
The inhibition of EV71 VP1 expression treated with different concentrations of compound, comparing to the expression of an internal control, GADPH.
Moreover, cell cycle arrest in SubG1 phase was observed upon treatment of A549 cells with 1 and 2 μM (IC50) concentrations of compound 11l and it induced apoptosis.
A biparametric cytofluorimetric analysis with FITC-labeled annexin V and propidium iodide showed induction of apoptosis by compound 11a at >1 μM concentrations of compound.
The cells were incubated at 37°C for 2.5 h, and then the concentrations of compound in the apical and basolateral side were quantitated by LC/MS/MS using standard analytical methods.
In addition, various surface morphologies and crystalline orientation of the plated copper films caused by different concentrations of compound 1b were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD) respectively.
Similar(8)
Very often the cells are extremely sensitive, enabling the animal to respond to very low concentrations of compounds.
Inhibitory concentrations of compounds were calculated and half maximal inhibitory concentrations (IC50) values were confirmed.
Table 2 Number of alive schizont at different concentrations of compounds 5a 5g Drug conc.
Briefly, 2 × MIC concentrations of compounds and drugs were selected for assay.
Sixteen hours later the cell cultures were treated with various concentrations of compounds 7f and 7g.
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