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The protein concentrations of cell lysate were determined using the protein assay dye reagent (Bio-Rad).
Following values were measured: The concentrations of cell mass and substrate in the medium as well as the oxygen and CO2 concentrations in exhaust gas, the local bubble size distribution (Sauter bubble diameter), the local gas hold up and the longitudinal concentration profiles of dissolved oxygen in the medium.
Dissolved sulfide concentrations of cell 2 effluent waters were typically > 1 mg L-1, sometimes as high as 30 mg L-1.
Medical flow cytometry (FACS) provides diagnostic answers by detecting the presence and concentration of cell populations, and/or by measuring concentrations of cell surface markers expressed on cells.
Concentrations of cell dry weight (CDW), glucose (GLC) and the AEC were measured offline, sampling after 5 residence times of one specific growth rate (0.1 ≤ μ (h−1) ≤ 0.7).
Protein concentrations of cell lysates were measured using a Protein Assay Kit II (BIO-RAD).
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The calculated concentrations of cell-released O2− vary from 6.772 to 24.652 pM cell−1 for the Zymosan amount used in this work.
a, b Different concentrations of cell-filtrate.
oryzae is similarly localized, the local concentrations of cell-wall-degrading enzymes are likely to be quite high during infection.
Protein concentrations of cell-lysates were determined using the micro bicinchonic acid kit (Pierce, Rockford, IL).
After a 48 h incubation, increasing concentrations of cell-free virus correlated with luciferase expression and therefore infection of TZM-bl cells (Fig. 6).
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