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As expected, a greater number of clusters of high concentrations of candidate mate choice genes occur on the X chromosome than on the autosomes (Fig. 2; Table S3).
An additional reason to assess plasma/serum concentrations of candidate CSF biomarkers is to determine if venipuncture, which is more easily performed than lumbar puncture, might yield equivalent information.
However, the tip of the left arm of chromosome III, which exerted a strong influence on mate choice patterns in previous studies using recombinant lines [22], also contains high concentrations of candidate mate choice genes in this study (Fig. 2; Table S3).
Concentrations of candidate biomarkers were assessed in serum samples from 133 women with breast adenocarcinoma and 119 healthy women.
The inhibition of attachment of 0.5 μg/ml of F4ac fimbriae to IPEC-J2 cells with various concentrations of candidate MFGM proteins was tested in Competitive ELISA.
Compared with Western blotting of the β-actin standard, the concentrations of candidate proteins in peritoneal dialysate samples were detected and analyzed by using the quantitative analysis software ImageQuant-TL-7.0.
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Table 2 presents the concentrations of candidate-IECs at which their production, under the applied experimental conditions, is no longer thermodynamically possible.
High-throughput determination of optimal concentrations of individual candidate attractants was successfully achieved through the binary choice assays in the semi-field enclosure.
The decrease in steady-state concentrations of other candidate mRNAs could also contribute to the alterations in cAMP metabolism that we observe.
QRT-PCR can also be used to verify transcript ratios in pools with variable concentrations of the candidate controls.
The serum concentrations of the candidate biomarkers were measured using a multiplex magnetic bead platform (MILLIPLEX™ MAP) designed for this project by the EMD Millipore Corporation (Billerica, MA, USA).
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