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DUBs were diluted to 2× indicated concentrations, mixed with substrate, and incubated for 15 min at 37°C.
Fig. 4 shows representative data traces for the normalised diameter for a range of diluent concentrations mixed with xanthan gum solution.
Synthetic L-γ-glutamylhydroxamate water solutions (0.5 ml), at desired concentrations, mixed with 0.5 ml reaction buffer and 0.5 ml of ferric chloride stop solution, were used for the construction of calibration curves.
This study was designed to analyze the reduction potential of three different liquid additives (water, rapeseed oil, and molasses) in three different concentrations mixed with cleaned whole or rolled oats on the generation of airborne particles under standardized laboratory conditions.
The positive clones or peptides at different concentrations mixed with HPV16E7 mAb were incubated with the coated E7 protein in microtiter wells for 3 h.
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These extreme amounts of nitrate and nitrite concentration mixed with water may occur through fertilizer runoff, animal wastes, leaking septic tanks, sanitary landfills, industrial waste waters, and discharge from car exhausts (Singh et al. 2014a, b).
An aqueous solution (150 ml) of sodium salt of humic acid (Loba Chemicals) at ppm concentration, mixed with 50 mg of ZnO/ZnO CuO catalysts was taken within the reactors and exposed to direct sunlight at noon for 3 h.
Viruses were then diluted to this concentration, mixed with two-fold serially diluted (1∶10 1∶1280) human serum in 96-well plates and incubated at 37 °C for 1 h.
Nine fractions (1.24 ml each) were harvested from the top of the tube (keeping aliquots to determine the sucrose concentration), mixed with 9 volumes of MBS, and centrifuged at 40,000 rpm for 1 h at +4°C (Beckman SW-41Ti rotor).
The TEV eluate was adjusted with CaCl2 to 3 mM final concentration, mixed with 6 ml of calmodulin-binding buffer 1 (10 mM β-mercaptoethanol, 10 mM Tris pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% NP-40, 1 mM imidazole, 1 mM Mg-Acetate, 2 mM CaCl2) and 150 μl calmodulin beads and incubated for 2 h at 4 °C on a rotating wheel.
Monosome and polysome fractions (each about 5 mL) were separated according to the live polysome profile and collected in ice-cold tubes containing 15 mL 6 M guanidine hydrochloride (resulting in ~4 M final concentration), mixed with 2.5 volumes ice-cold 100% ethanol and precipitated o/n at −20°C.
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