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With dye concentrations maintained at 30 mg L− 1, a 22 central composite design (CCD) was applied whose variables of interest were pH and flow.
Cell number and chondrogenic phenotype (CD14 CD90 ratios) were found to decline in regions with higher RGD concentrations, while regions with lower RGD concentrations maintained cell number and phenotype.
Coenzymes are usually continuously regenerated and their concentrations maintained at a steady level inside the cell.
In assays where metabolic inhibitors were used, these were added to cell monolayers for 60 min prior to the experiment and concentrations maintained during incubation with bacteria.
Consequently, successful root uptake and assimilation of peptides when supplied at the low concentrations maintained in soil, strongly suggests that plants are capable of competing with soil microorganisms for N at an early stage of protein decomposition.
For experiments using inhibitors of cell function, these were incubated with the cells 60 min prior to the addition of bacteria and concentrations maintained during the assay; Genistein (200 µM), Wortmannin (20 nM), cytochalasin D (50 µM), PP2 (10 µM), cholchicine (1 µM), cycloheximide (25 µM) and methyl-β-cyclodextrin (2 mM) [17].
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Fiber reduced significantly the number of peaks observed in the levodopa concentrations, maintaining concentrations more stable.
Haematocrit-standardized concentrations maintain a stable ratio with therapeutically active unbound concentrations (assumed proportional to Cp) no matter what the haematocrit fraction.
This inhibition leads to the prolonged activity of cGMP which, in turn, reduces intracellular calcium concentrations, maintains smooth muscle relaxation, and results in rigid penile erections.
The 2-CP concentration maintained a constant level which represented the steady-state condition.
The working condition was set at 37 °C; initial pH, 7.0; air flow, 1.5 vvm; and dissolved oxygen concentration (maintained through agitation), 20%.
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