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cDNA libraries for PolyRibo-3′ RNA-sequencing was performed using identical concentrations from each of the RNA sample fractions collected after polysome fractionation that was performed as indicated above.
Samples with different concentrations from each copolymer (0.5%, 1%, 1.5%, 2%, 2.5%, 3% by weight) were prepared and evaluated as viscosity index improvers for lube oil.
Total mRNA concentrations from each sample were determined using a spectrophotometer (NanoDrop 2000c, Thermo Scientific, Wilmington, DE, USA).
Protein concentrations from each sample were measured by BCA method (Pierce Biotechnology Inc., Rockford, IL, USA).
The mean of the two PRL concentrations from each dog was used for statistical analysis.
Optimisation experiments showed that different protein concentrations from each region were within the linear range of the detection system.
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Monthly air pollution dispersion modeling (1985 1994) (based on individual industrial source processing-plant engineering specifications, emission volumes, and meteorologic information) provided exposure isopleths of sulfur dioxide concentration from each of 231 sour-gas processing-plants across the province.
In order to normalize the levels of the mouse Prpf3 protein, we measured the total protein concentration from each sample and loaded equal amounts of protein (100 µg) on SDS-PAGE followed by either immunoblotting or Coomassie Brilliant Blue staining.
The oxygen concentration from each chamber was sampled at 1 Hz for 15 minutes every two hours and during this period fH was recorded simultaneously by the attached data logger (see above).
To determine glucose uptake rates, 20 µM glucose was added to the Krebs Ringer buffer and 30 µL aliquots were taken at 0, 15, 15, 30, 60 and 120 min. Glucose concentration from each aliquot was determined using a fluorescent glucose assay kit (BioVision, # K606-100) as per manufacturer's instructions.
The data obtained were normalized to the protein concentration from each well.
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