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After transplanting seedlings in vermiculite a nitrogen gradient was simulated by applying complete micronutrient solutions with nitrate concentrations fixed at 0, 0.5, 1, 2 and 4 mM NO3−, all other elements applying to nitrogen-free nutrient solution concentrations [44].
Next, we assessed tyrosine, tyrosine methyl ester, and tryptophan as AANATA inhibitors at a concentration of 1.0 mM, with the acetyl-CoA (39 μM) and tyramine (12 μM) concentrations fixed at their respective Km,app values.
To extend the binding detection range, we set to modify the NMR experimental conditions by first increasing the protein concentration to 40 μM while maintaining ligand concentrations fixed at 1 mM (setup 2); and second by increasing concentrations of protein and ligand to 30 μM and 3 mM (set-up 3), respectively, thereby maintaining a protein/ligand excess of 100-fold as in set-up 1.
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Michaelis-Menten model still can be used in the present reaction by fixing the D-isoascorbic acid concentration fixed at 2.5 mM [30].
Six (6) compositions of the mixed solvent with varying concentrations of IL and MEA concentration fixed at 30 wt% for all cases were evaluated through process simulations.
As shown in Table 5, with the surfactant concentration fixed at 0.15 %, when the polymer concentration increased the large pore contribution did not change significantly, and the medium pore contribution increased while the small pore contribution decreased.
The degree of synergism between artemisinin and vincristin was determined by using CI analysis at a non-constant ratio, i.e., drug combinations were made by varying the concentrations of one drug (vincristin) while keeping the second drug (artemisinin) concentration fixed at IC30.
We validated the quality of our response-surface models in a second experiment in which we kept KCl concentration fixed at 90.6 mM while varying pH values and Fructose 1,6-bisphosphate concentrations in eighty mixtures according to a space-filling design.
(B ) Specific activity of ParA (concentration fixed at 6 µM) measured as a function of DNA concentration (0 2.0 mg/ml).
To further verify this interaction, we performed a titration experiment of zeta potential with GO concentration fixed at 5 μg/mL.
The initial conditions were pH 7.00, temperature 50.00°C, E/S 0.01, incubation period 10.00 h (the substrate concentration fixed at 1.00%).
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