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Maximal antiangiogenic activity typically requires prolonged exposure to low drug concentrations, exactly counter to maximum tolerated doses administered when optimal tumor cell kill is the goal.
We calculated the loss of Cdc42 (initial concentration 300 μ M) with time for different Bem1-GEF-Cdc42 concentrations exactly as we did for Bem1-GEF, and plotted the resulting dwell times for varying GEF concentration.
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Further, we were interested in TCEP concentration, exactly in the most suitable TCEP concentration for MT determination.
However, even with ideal dose adjustment with an average concentration exactly on target, 35% of concentrations in a typical patient will be outside the suggested acceptable range.
"It turns out these enhanced concentrations are exactly opposite each other they're antipodal," says Matthew Siegler, a planetary scientist at the Planetary Science Institute who is based in Dallas, Texas.
These results mean although the initial Zn2+ ions concentrations are exactly the same, while the different absolute quantities of Zn2+ ions (different supersaturation degree) do affect the growth of ZnO nanorods.
These concentrations correlated exactly to the LD 50 levels in the embryos (Table 5 and Figure 3A).
However, lichenysin was not commercially available at the time, hence Qiu et al. (2014) determined the lichenysin concentration as exactly as possible.
Use of the Bischoff-Aris general modulus allowed the analytical proof that apparent coefficients for immobilized enzymes following Michaelis-Menten kinetics, under strong intraparticle diffusion control, whose deactivation was unaffected by reactant concentration, were exactly half those of enzymes not under diffusion limitation.
But Hoechst concentration is exactly the parameter that mostly differs among published data.
Total RNA was extracted using RNA Blue kit (Top-Bio, Czech Republic) and the concentrations were leveled exactly to 1 µg µL−1 by DEPC-treated water in all samples.
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