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The mean annual concentrations estimated at the home addresses reported in the follow-up questionnaire were used as a proxy of the children's outdoor residential exposure.
The WHO guidelines indicate that the lower range of concentrations at which adverse health effects have been demonstrated is not greatly above background concentrations (estimated at 3 5 μg/m in the United States and Western Europe for PM2.5).
ELISAs were performed in 96-well plates following the manufacturer's instructions using IL-6 and TNF Duoset kits (DY 206, DY210 R&D Systems), with protein concentrations estimated at 450 nm from a standard calibration curve included on each plate.
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Typically, under normal physiological conditions, H2O2 has an extracellular concentration estimated at 10− 7~10− 6 M, which is about 10-fold higher than that observed in intracellular fluid [1, 41, 42].
The ice concentration is computed daily using a daily RADARSAT-2 SAR mosaic as its input, and it thus gives the concentration estimated at each grid cell (pixel) based on the most recent SAR data at the location.
Open image in new window Fig. 8 Ratio of the chloride concentration estimated at the depth of rebars in the concrete matrix (x or ( rho_{1} ) = 4 cm) C and the chloride threshold concentration for the beginning of the depassivation ( C_{Cr} ) as function of the exposure time t.
We also used them in the current analysis to examine characteristics of the automated method by generating point concentration estimates at the water sampling locations using estimated values for initial concentration of PCE in the pipe and residential water use.
(D ) Assessment of variability in the concentration estimates at a fixed position of the center of the 1.0 M odor source (15 mm).
The PDE model leads to a good fit of the temporal profiles of the average concentrations after an initial transient phase of 30 s. (C ) Assessment of variability in the concentration estimates at a fixed position of the center of the 1.0 M odor source (7.5 mm).
Protein identity was confirmed by ESI-TOF mass spectrometry and concentrations estimated by UV at 280 nm.
Shortly, cells were plated in triplicates in 60 mm dishes (Nunc Thermo Fisher, Waltham, MA, USA) at concentrations estimated to yield 40 150 colonies per dish.
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