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Samples were concentrated using a Macrosep® ultrafiltration device (Pall life sciences, USA) and protein concentrations determined with a BCA protein detection kit (Pierce , USA, according to the manufacturer's instructions.
Immediately thereafter, each mouse was sacrificed, choroid plexus isolated (n = 4), and the ion concentrations determined with flame photometry (Na+ and K+) or by colorimetry (Cl−).
The mycoproteins were extracted with ultrasonication and their concentrations determined with Coomassie Brilliant Blue.
Aspergillus niger cell-free extracts were prepared as described by Kobayashiet al. (2013), and the protein concentrations determined with a Bradford Protein Assay Kit.
Blood samples were collected by cardiac puncture immediately following euthanasia and serum immunoglobulin E (IgE) concentrations determined with a mouse IgE enzyme-linked immunosorbent assay (ELISA) quantification kit (Bethyl Laboratories, Montgomery, TX, USA) according to the manufacturer's protocol [19].
In contrast, the concentrations determined with the phantom replacement method were clearly erroneous when in vivo coil loading conditions were not properly replicated during the calibration session.
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MBCstat: minimal bactericidal concentration determined with stationary-phase bacteria.
Suspensions were filtered through cheesecloth and the spore concentration determined with a hemacytometer.
Ligated fragments of interest were amplified by PCR, gel purified and their concentration determined with a Nanodrop spectrophotometer.
The volume of urine was measured, and albumin concentration determined with mouse albumin ELISA kit (CellTrend, Luckenwalde, Germany).
Cells were lysed using cell lysis buffer (Cell Signaling) and a protein concentration determined with the BCA Protein Assay kit (Pierce).
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