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Both Ku and TKu were concentrated and concentrations determined using the Micro BCA Protein Assay Kit (Pierce) using BSA as standard and further confirmed by UV absorbance.
Prata, A. J. & Prata, A. T. Eyjafjallajökull volcanic ash concentrations determined using Spin Enhanced Visible and Infrared Imager measurements.
The ignition delay time was determined from three different measurements at the sidewall: broadband chemiluminescent emission captured via a photodetector, CH4 concentrations determined using a distributed feedback interband cascade laser centered at 3403.4 nm, and pressure recorded via a dynamic Kistler type transducer.
Great care needs to be exercised in correlating hepcidin concentrations determined using different methods.
Two independent validations by high-performance liquid chromatography (HPLC) and histologic fluorescence in a rat fibrosarcoma (FSA) model indicate a concordant linear relationship between DOX concentrations determined using T(1) and those measured invasively.
At 48 h post transfection, whole cell lysates were prepared and their protein concentrations determined using the procedures described above (for Western blotting analysis).
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The cell concentration determined using a CASY-1 Coulter Counter.
Protein concentrations were determined using the BCA Protein Assay Kit Thermo Fisher Scientificc).
Protein concentrations were determined using the BCA Protein Assay Kit Pierce Biotechnology Rockfordrd, IL, USA).
Antibody concentrations were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific).
Plasma tocotrienol concentrations were determined using HPLC method.
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