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The overall removal efficiency of CWs was dependent mainly on inflow pollutant concentrations, design, maintenance parameters and operating conditions.
Two original designs, in which two of the precursors concentrations vary, are built to estimate the parameters (or effects) of two predictive quadratic models giving the strontium and lanthanum proportions vs. strontium and manganese concentrations (design 1) and vs. strontium and lanthanum concentrations (design 2), the third precursor concentration being fixed.
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Fumigations were conducted in solardomes with diesel exhaust pollutants at concentrations designed to simulate those close to a major highway in inner London.
We have used these cells to evaluate, using immunocytochemistry, reverse transcriptase-polymerase chain reaction, and Western blotting, direct effects of the glucocorticoid dexamethasone at concentrations designed to mimic in vivo therapeutic corticosteroid levels.
The 8 drugs were ranked according to their in vivo effect at doses equitoxic to mice, and according to their in vitro effect at concentrations designed to approximate to levels of drugs in human plasma.
Odors were presented as mixtures in mineral oil (octanal [0.31%], heptanal [0.068%], eugenol [2.65%]) or propylene glycol (lyral [10%]) continuously for 21 days at concentrations designed to achieve a vapor pressure of approximately 1 Pa per odorant.
We evaluated insulin sensitivity during basal postabsorptive conditions and across a physiological range of plasma insulin concentrations designed to simulate those observed during postprandial conditions in lean and obese subjects who had normal oral glucose tolerance.
A similar approach is the predictive 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) assay, in which dissected tumour biopsies of approximately 1 mm3 are cultured with chemotherapeutic drugs at concentrations designed to mimic the plasma levels achieved in vivo [ 48].
The concentration performance was further improved by using a two-stage concentration design.
In doing so, the use of a multi-concentration design increases the robustness of the test results.
It contains the functions spoD, setD, and doseD which cover identification of the spontaneous lethality, the estimation of the necessary number of test organisms, and a concentration design according to the user's requirements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com