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The increase of plasma urea concentrations continued for a longer time after restricted feed intake in WB-reindeer, and three of the reindeer that died (Nos. 13, 33 and 69) had urea levels between 17 and 22 mmol l-1 prior to death.
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Initial observations indicated this low concentration continued for some time after K was last applied.
was added as indicated concentrations, and incubation continued for 5 hr.
24 hr later, bortezomib was added at the specified concentrations and incubation continued for 48 hr.
Measurement of aqueous cyanide concentrations continues to be a pressing challenge for both industrial processes and waste solutions.
Equal numbers of viable cells (5 × 10 cells per well) were incubated in a total volume of 100 μl of RPMI 1640 medium supplemented with 10% FCS alone or with Atiprimod at increasing concentrations; the incubations were continued for 72 h in 96-well flat-bottomed plates (Linbro; Flow Laboratories, McLean, VA, USA) at 37°C in a humidified 5% CO2 atmosphere.
After 15 min, non-radioactive ATP was added to 2.5 mM final concentration and reactions were continued for an additional 15 minutes to ensure complete 5'-phosphorylation.
Two days prior to culture termination on days 3, 5, and 7, the media was replaced with media containing S-H2SO4 (Perkin-Elmer Life and Analytical Science) at a final SO4 well concentration of 5 μCi/ml together with the same concentrations of PPS and culture continued for a further 2 days.
LPS also induced an increase in plasma FFA concentrations within 1 h, which continued for 24 h, consistent with a potent lipolytic effect of LPS.
Glycine was added at 125 mM final concentration and the incubation was continued for 5 min to stop the fixing reaction.
Overproduction of the recombinant protein was initiated by the addition of isopropyl thio-β d-galactoside to a final concentration of 1 mM and continued for 3 h at 37°C.
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