Sentence examples for concentrations concentrated using from inspiring English sources
The constructs were eluted with a 0 500 mM gradient of imidazole concentrations, concentrated using a 5 kDa-cutoff concentrator (Millipore) and dialyzed against 10 mM Tris-HCl, pH 8.0, containing 200 mM NaCl.
The resulting Nextera XT libraries were very low concentration, so after quantification by Qubit HS DNA assay, were pooled to give a 0.1 nM concentration and concentrated using a SpeedVac.
Combined protein-containing fractions identified by SDS PAGE were concentrated using centrifugal concentrations (Amicon, 10 kDa MWCO) and applied to a Hi-Load 26/60 Superdex 75 size-exclusion column using an Akta Purifier FPLC system.
The fractions from each NaCl concentration were desalted and concentrated using Amicon Ultra-4 centrifugal filters (Millipore).
In order to deliver more scFv-L-Aff for enhancing the efficiency of pretargeting, the fusion protein was concentrated using centrifugal concentration.
The filtrate was concentrated using vacuum concentrator.
It was then concentrated using 10 kDa concentration columns (Millipore Billerica, MA) and subsequently analyzed using the ELISA kit.
rRNA-depleted total RNA was concentrated using the Ribominus concentration module (Life Technologies, CA) and assessed on the Agilent 2100 Bioanalyzer for the confirmation of rRNA removal.
To obtained high concentrations for characterization, the proteins were concentrated using a spin column with MWCO 3,000 Da (Millipore).
If required, samples were concentrated using a Savant SpeedVac concentrator (GMI) to achieve a final concentration of about 100 ng/µl.
Viral supernatant was harvested 48 hours after transfection, concentrated using Peg-it Virus Concentration solution (System Biosciences, Mountain View, CA, USA) and the titer was assessed.
