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We tested this relationship at higher concentrations, calculating the relative EMS induced mutation rates at 0 mM EMS, 40 mM EMS, 80 mM EMS, and 100 mM EMS by measuring the reversion rate of C. elegans mutants exposed to each EMS concentration.
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The lower graph represents the sulfide concentrations calculated for a pH=6.5.
Overall, the effective concentrations calculated using the model correspond very well with the observed amount of enzyme inhibition (Supplementary Fig. 6).
The agent concentrations calculated from equations (3a) and (3b) were then compared with known concentrations in each phantom using Pearson correlations with a probability of p < 0.05 used as a determination for significance.
Values were plotted in dose-response curves and, if applicable, CC50 concentrations calculated.
Actin polymerization assays were performed and barbed end concentrations calculated as previously described (22).
The metabolite concentrations calculated using the ERETIC reference signal are presented in Table 3.
The gray circles at time zero indicate the initial cell concentrations calculated from inoculation procedure.
Consequently, estimated average glucose concentrations calculated from measured HbA1c values should be used with caution.
The millimolar concentration calculated in the present study is consistent with concentrations found in other algal cells (between 1 and 15 mmol P L−1)42,43,44,45.
Furthermore, the in vivo 50% effective concentration calculated from these data best matched GSK501015's in vitro potency when serum protein binding was accounted for.
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