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In the case of quantitation of target metabolites, each region of the NOESY spectra corresponding to a metabolite signal was selected and integrated in Amix using the Variable Sized Bucketing module and concentrations calculated with respect to the 1 mM TSP internal standard.
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At termination absorbance was quantified (595 nm) and concentration calculated with reference to standard curve.
26 We compared the measured arterial oxygen concentrations with the concentrations calculated from pulse oximetry at the time of the blood gas sample.
All kits were read using a Luminex 100 IS System (Luminex Corp, Austin, TX) by the Rush Proteomics and Biomarkers Core Facility with biomarker concentrations calculated using a 5-parametric curve fit using xPonent 3.2 software (Luminex Corp).
Equation 1 was adapted into Equations 2 and 3 to separately track indoor pollutants originating from indoor emissions (Cin_I,i) and indoor pollutants from outdoor sources (Cin_O,i,) with total concentrations calculated as the summed contributions from the two sources (Equation 4).
Relative concentrations were calculated with respect to acyl-CoAs concentrations in Apc Wild-Type Low Fat (WT-LF) animals.
Additional Cu2+ concentrations were calculated with the ligand data and the dissolved copper concentrations determined from ambient (pier) samples.
Permeate alcohol concentrations are calculated with the help of known separation factors and feed alcohol concentrations.
IR concentrations are calculated with two different A values (cm−2).
Melatonin concentrations were calculated with external analytical curves (minimum of 5 points).
Mean standard deviation and coefficient of correlation (r value) of soil and crop parameters with effluent concentrations were calculated with MS Excel (ver. 2003, Microsoft Redmond Campus, Redmond, WA) and graphs produced with Sigma plot (ver. 12.3, Systat Software, Inc., Chicago, IL).
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