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Actin polymerization assays were performed and barbed end concentrations calculated as previously described (22).
Fluorescence was excited at 340 and 380 nm, emission recorded at 22 24 Hz with a 14-bit EMCCD camera (iXON3 885, Andor, Belfast, UK), and Ca2+ concentrations calculated as described previously (Grynkiewicz et al, 1985).
Equation 1 was adapted into Equations 2 and 3 to separately track indoor pollutants originating from indoor emissions (Cin_I,i) and indoor pollutants from outdoor sources (Cin_O,i,) with total concentrations calculated as the summed contributions from the two sources (Equation 4).
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For each RNA sample, absorption at 260 nm was measured and RNA concentration calculated as A260 × 40 (μg/mL) × dilution factor.
Figure 2 shows that the relation between intake and plasma concentration, calculated as the concentration divided by the dose, for soy foods is not different than that for isoflavone supplements.
Model 6, which does not require direct measurements in individual subject microenvironments, predicts the personal concentration considering the PE concentration calculated as in model 5 and incorporating add-on variables extracted from the questionnaires that explain additional factors.
Kd and maximum analyte binding (Bmax) values were obtained by non-linear curve fitting of a 1 1 Langmuir interaction model (bound= Bmax/ Kd+ C), where C is the analyte concentration calculated as monomer).
Relative analyte recovery values were calculated as: measured concentration × 100/spiked-in concentration.
The relative concentration difference, calculated as ((venous concentration-arterial concentration)/arterial concentration) in the 240 minute post-dose sample (latest sample for which both sample matrices were available) was always less than 1 ng/mL.
Relative PAH concentrations were calculated as the ratio of PAH concentration to the total PAH concentration.
Coarse particle (2.5 < aerodynamic diameter < 10 μm) concentrations were calculated as the difference between PM10 and PM2.5 concentrations.
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