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In the Leishmania Antigen ELISA, antigen concentrations are calibrated from a standard curve with known concentration of antigens and expressed as Urine Antigen Units (UAU /mL (Fig. 3a).
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Hourly SAT glucose concentrations were calibrated to arterial blood glucose (Bg) by one-point calibration either 1 hour (BgSAT1h) or 6 hours (BgSAT6h) after catheter insertion.
Isotope ratios and elemental concentrations were calibrated using the Curtin University standard CZ3 (564 Ma 206Pb/238UU = 0.0914, 550 ppm U), and data were reduced using Krill software (developed by P. Kinny).
These concentrations were calibrated prior to the experiment and were the highest doses that did not harm the animals' weight or their general well being (not shown).
All exposure concentrations were calibrated to be equipotent based on the yeast estrogen screen (YES), which reports activation of hERα.
These concentrations were calibrated as 109 kBq ml−1, 164 kBq ml−1 and 218 kBq ml−1 at mid-scan time.
The purity of the collected products was confirmed by RP-HPLC and ESI-MS, and the conjugate concentrations were calibrated before being aliquotted into predetermined amounts.
All purified proteins used in the present study were quantified by UV absorption and the final concentrations were calibrated by SDS/PAGE as shown in Supplementary Figure S1.
The anticipated range of fractional gas concentrations was calibrated before each trial with 15.8% O2 and 5% CO2 in N2 (Praxiar Technology Inc., Danbury, CT, USA).
Serum creatinine concentrations were calibrated to the assays used for the development of the modification of diet in renal disease (MDRD) equation (19).
Therefore, real-time concentrations were calibrated based on the average PM2.5 mass concentration obtained from the gravimetric analysis each day to ensure a more reliable measure of individual exposure [see Supplemental Material available online (doi 10.1289/ehp.0900818.S1 via http://dx.doi.org/)].
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